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Our approach to ameliorate is reproducible and could enhance the sensitivity and specificity of the assembled transcriptome which could be critical for validation of the assembled transcripts and for planning various downstream biological assays. We found that, primary transcriptome assembly obtained as a result of Trinity can be ameliorated on the basis of transcript length, coverage, and depth and protein homology. In this study, we aimed at obtaining an ameliorated de novo transcriptome assembly with sequence reads obtained from Illumina platform and assembled using Trinity Assembler. Various de novo transcriptome assemblers are also available today for construction of de novo transcriptome. Among various sequencing platforms available, Illumina is the most widely used platform based on data quality, quantity and cost. Retrieved 21 November 2014.Advent of Next Generation Sequencing has led to possibilities of de novo transcriptome assembly of organisms without availability of complete genome sequence. The software program application also contains superior safety in competition to malware spread through e-mail or instant messaging.
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